Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms.
Identifieur interne : 000016 ( Main/Exploration ); précédent : 000015; suivant : 000017Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms.
Auteurs : Sabrina Boumaiza [Algérie] ; Habiba Oussedik-Oumehdi [Algérie] ; Fatima Laraba-Djebari [Algérie]Source :
- Biologicals : journal of the International Association of Biological Standardization [ 1095-8320 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- antagonistes et inhibiteurs : Venins de vipère.
- pharmacologie : Sérums antivenimeux.
- toxicité : Venins de vipère.
- Animaux, Radio-isotopes du cobalt, Rayons gamma, Souris, Sérums antivenimeux, Viperidae.
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : Viper Venoms.
- chemical , chemistry : Antivenins.
- chemical , pharmacology : Antivenins.
- chemical , toxicity : Viper Venoms.
- Animals, Cobalt Radioisotopes, Gamma Rays, Mice, Viperidae.
Abstract
Cerastes cerastes and Vipera lebetina are the most medically important vipers in Algeria. Their bite induces several pathological effects on victims of accidental envenomation. In this study we analyzed the pathogenesis induced after an experimental envenomation. Indeed, we determined, in vitro, venom enzymatic activities and we analyzed, in vivo, pathological effects induced on liver, heart, lung and skin. In addition we investigated the neutralizing potency of four experimental antivenoms elicited against native and irradiated venoms. Results revealed that V. lebetina and Cerastes cerastes venoms presented strong hemorrhagic, oedematic and necrotic activities. Histopathological study showed that both venoms induced deep damage in tissue structures leading to organ dysfunction. They also increased cellular peroxidases activities, indicating an inflammatory process that is known to amplify tissue damage. Western-blot analysis evidenced that anti-irradiated venoms recognized most components of native venoms. Antivenoms were effective in neutralizing all tested activities, with an increased protective effect obtained with anti-irradiated venoms. Anti-irradiated venoms reduced cellular peroxidases activities indicating a reduction of the inflammatory response. These results may improve our understanding of Algerian Viperidae bite pathogenesis and would encourage further studies planning to provide more proofs on the effectiveness of anti-irradiated venoms administration in the treatment of envenomation.
DOI: 10.1016/j.biologicals.2015.11.002
PubMed: 26678662
Affiliations:
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Their bite induces several pathological effects on victims of accidental envenomation. In this study we analyzed the pathogenesis induced after an experimental envenomation. Indeed, we determined, in vitro, venom enzymatic activities and we analyzed, in vivo, pathological effects induced on liver, heart, lung and skin. In addition we investigated the neutralizing potency of four experimental antivenoms elicited against native and irradiated venoms. Results revealed that V. lebetina and Cerastes cerastes venoms presented strong hemorrhagic, oedematic and necrotic activities. Histopathological study showed that both venoms induced deep damage in tissue structures leading to organ dysfunction. They also increased cellular peroxidases activities, indicating an inflammatory process that is known to amplify tissue damage. Western-blot analysis evidenced that anti-irradiated venoms recognized most components of native venoms. Antivenoms were effective in neutralizing all tested activities, with an increased protective effect obtained with anti-irradiated venoms. Anti-irradiated venoms reduced cellular peroxidases activities indicating a reduction of the inflammatory response. These results may improve our understanding of Algerian Viperidae bite pathogenesis and would encourage further studies planning to provide more proofs on the effectiveness of anti-irradiated venoms administration in the treatment of envenomation.</div></front></TEI><affiliations><list><country><li>Algérie</li></country><region><li>Wilaya d'Alger</li></region><settlement><li>Alger</li></settlement></list><tree><country name="Algérie"><region name="Wilaya d'Alger"><name sortKey="Boumaiza, Sabrina" sort="Boumaiza, Sabrina" uniqKey="Boumaiza S" first="Sabrina" last="Boumaiza">Sabrina Boumaiza</name></region><name sortKey="Laraba Djebari, Fatima" sort="Laraba Djebari, Fatima" uniqKey="Laraba Djebari F" first="Fatima" last="Laraba-Djebari">Fatima Laraba-Djebari</name><name sortKey="Oussedik Oumehdi, Habiba" sort="Oussedik Oumehdi, Habiba" uniqKey="Oussedik Oumehdi H" first="Habiba" last="Oussedik-Oumehdi">Habiba Oussedik-Oumehdi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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